gata4 specific polyclonal antibody  (Proteintech)

Journal: Cell Proliferation

Article Title: Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy

doi: 10.1111/cpr.13052

Figure Lengend Snippet: All antibody lists

Article Snippet: GATA4 , Rabbit polyclonal , 1:1000 for WB , Proteintech, China.

Techniques:

Journal: Scientific Reports

Article Title: The Synthetic Curcumin Analogue GO-Y030 Effectively Suppresses the Development of Pressure Overload-induced Heart Failure in Mice

doi: 10.1038/s41598-020-64207-w

Figure Lengend Snippet: GO-Y030 at a dose 1/10th that of curcumin significantly suppressed PE-induced hypertrophic responses in cardiomyocytes. Primary cultured cardiomyocytes were treated with 3 or 10 μM curcumin, or with 0.3 or 1 μM GO-Y030 and were stimulated with 30 μM PE. (a) Histone fractions isolated from these cells were subjected to western blotting using anti-acetyl-histone H3 (Lys9) antibodies and anti-histone H3 antibodies. Full-length blots are presented in Supplementary Fig. . (b) The levels of acetylated histone H3K9 and total histone H3 were quantified. The data are presented as the mean ± SEM of three individual experiments. (c,d) Total RNA was extracted from the cells, and quantitative PCR was performed for ANF (c) , BNP (d) , and 18S. The data are presented as the mean ± SEM of three individual experiments. (e) Immunofluorescence staining was performed using anti-MHC antibodies and Alexa Fluor 555-conjugated anti-mouse IgG. Scale bar: 20 μm. (f) The surface area of these cells was measured using ImageJ software. All data are presented as the mean ± SEM of three individual experiments. (g) Nuclear extracts prepared from primary cultured cardiomyocytes were subjected to western blotting with anti-p300 antibodies, anti-GATA4 antibodies, and anti-β-actin antibodies. Full-length blots are presented in Supplementary Fig. . (h) The nuclear extracts were immunoprecipitated with goat anti-GATA4 polyclonal antibodies and subjected to western blotting with anti-p300 antibodies, anti-acetyl-lysine antibodies, and anti-GATA4 antibodies. The original images are presented in Supplementary Fig. . (i) The levels of acetylated GATA4 were quantified. The data are presented as the mean ± SEM of three individual experiments.

Article Snippet: For western blotting, rabbit polyclonal antibodies against acetylated lysine (Cell Signalling), mouse anti-GATA4 polyclonal antibodies (Cell Signalling), rabbit anti-p300 polyclonal antibodies (Santa Cruz Biotechnology Inc.), and mouse anti-β-actin monoclonal antibodies (Sigma-Aldrich, USA) were used.

Techniques: Cell Culture, Isolation, Western Blot, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining, Software, Immunoprecipitation

Journal: eLife

Article Title: Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4

doi: 10.7554/elife.48767

Figure Lengend Snippet: Figure 1. Induction of Sertoli-like cells (hiSCs) from human fibroblasts. (A) Immunostaining of NR5A1, GATA4, WT1, SOX9 and DMRT1 (red signal) in fibroblasts (HPF) 3 days post infection. DAPI (blue signal) was used to indicate nucleus. Scale bar = 25 mm. NC represents HPF stained with only secondary antibody. (B) Experimental design for the reprogramming of human Sertoli-like cells (hiSCs). Human fibroblasts were infected by lentivirus carrying human transcription factors: NR5A1, GATA4, WT1, SOX9 and DMRT1 (5TFs). The cells were cultured in MEF medium for 1 day after infection Figure 1 continued on next page

Article Snippet: DOI: https://doi.org/10.7554/eLife.48767 18 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-human CX43 (Rabbit polyclonal) Cell Sigaling Cat#: 3512S IF(1:100) Antibody anti-human DMRT1 (Rabbit polyclonal) Abcam Cat#: Ab1786 IF(1:100) Antibody anti-human WT1 (Rabbit polyclonal) Proteintech Cat#: 12609–1-AP IF(1:100) Antibody anti-human GATA4 (Rabbit polyclonal) Proteintech Cat#: 19530–1-AP IF(1:100) Antibody anti-human NR5A1 (Rabbit polyclonal) Proteintech Cat#: 18658–1-AP IF(1:100) Antibody donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#: A-21202; RRID: AB_141607 IF(1:1000) Antibody donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#: A-31572; RRID: AB_1567203 IF(1:1000) Strain, strain background (Escherichia coli) TransStbl3 Chemically Competent Cell TransGen Biotech Cat#: CD521 Peptide, recombinant protein bFGF, Recombinant Human FGF basic Protein R and D Systems Cat#: 233-FB-CF Chemical compound, drug G418, Geneticin Thermo Fisher Scientific Cat#: 10131035 Chemical compound, drug Blasticidin Thermo Fisher Scientific Cat#: R21002 Software, algorithm, website ImageJ NIH https://imagej.nih.gov/ij/ Software, algorithm, website FlowJo (v10.3) BD https://www.flowjo.com Software, algorithm, website Prism7 (v7.0 a) Graphpad Software https://www.graphpad.com/ scientific-software/prism/ Software, algorithm DAVID Bioinformatics Resources (v6.8; GO) https://david.ncifcrf.gov Software, algorithm, website Tophat2/cufflinks (Kim et al., 2013) http://ccb.jhu.edu/ software/tophat Software, algorithm, website R (v3.4.1; PCA, cluster and DEG) https://www.

Techniques: Immunostaining, Infection, Staining, Cell Culture

Journal: eLife

Article Title: Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4

doi: 10.7554/elife.48767

Figure Lengend Snippet: Figure 3. NR5A1 and GATA4 are sufficient to derive hiSCs. (A) Representative FACS results of different combinations of NR5A1, GATA4, WT1, SOX9 and DMRT1 for the induction of AMH:EGFP+ cells. dH1 fibroblasts were transduced with the indicated factors and reprogrammed for 10 days. The combinations were divided into three groups: -NR5A1, combinations without NR5A1; +NR5A1, combinations with NR5A1 but without GATA4; +NR5A1 and GATA4, combinations with both NR5A1 and GATA4. (B) Quantitative data of EGFP+ cells in (A). n = 2, biological replicates, error bar indicates SD, Figure 3 continued on next page

Article Snippet: DOI: https://doi.org/10.7554/eLife.48767 18 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-human CX43 (Rabbit polyclonal) Cell Sigaling Cat#: 3512S IF(1:100) Antibody anti-human DMRT1 (Rabbit polyclonal) Abcam Cat#: Ab1786 IF(1:100) Antibody anti-human WT1 (Rabbit polyclonal) Proteintech Cat#: 12609–1-AP IF(1:100) Antibody anti-human GATA4 (Rabbit polyclonal) Proteintech Cat#: 19530–1-AP IF(1:100) Antibody anti-human NR5A1 (Rabbit polyclonal) Proteintech Cat#: 18658–1-AP IF(1:100) Antibody donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#: A-21202; RRID: AB_141607 IF(1:1000) Antibody donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#: A-31572; RRID: AB_1567203 IF(1:1000) Strain, strain background (Escherichia coli) TransStbl3 Chemically Competent Cell TransGen Biotech Cat#: CD521 Peptide, recombinant protein bFGF, Recombinant Human FGF basic Protein R and D Systems Cat#: 233-FB-CF Chemical compound, drug G418, Geneticin Thermo Fisher Scientific Cat#: 10131035 Chemical compound, drug Blasticidin Thermo Fisher Scientific Cat#: R21002 Software, algorithm, website ImageJ NIH https://imagej.nih.gov/ij/ Software, algorithm, website FlowJo (v10.3) BD https://www.flowjo.com Software, algorithm, website Prism7 (v7.0 a) Graphpad Software https://www.graphpad.com/ scientific-software/prism/ Software, algorithm DAVID Bioinformatics Resources (v6.8; GO) https://david.ncifcrf.gov Software, algorithm, website Tophat2/cufflinks (Kim et al., 2013) http://ccb.jhu.edu/ software/tophat Software, algorithm, website R (v3.4.1; PCA, cluster and DEG) https://www.

Techniques: Transduction